Metsi a se Nang Endotoxinvs Ultrapure Metsi: Ho Utloisisa Liphapang tsa Bohlokoa
Lefats'eng la lipatlisiso le tlhahiso ea laboratori, metsi a bapala karolo ea bohlokoa lits'ebetsong tse fapaneng.Mefuta e 'meli e sebelisoang hangata ea metsi litlhophisong tsena ke metsi a se nang endotoxin le metsi a ultrapure.Le hoja mefuta ena e ’meli ea metsi e ka ’na ea bonahala e tšoana, ha e tšoane.Ha e le hantle, ho na le phapang e ka sehloohong pakeng tsa tse peli tseo e leng tsa bohlokoa ho utloisisa e le ho netefatsa katleho le ho nepahala ha liphello tsa liteko.
Sehloohong sena, re tla hlahloba phapang lipakeng tsa metsi a se nang endotoxin le metsi a ultrapure, mme re buisane ka ts'ebeliso ea ona le bohlokoa ba ona tikolohong ea laboratori.
Metsi a se nang endotoxin ke metsi a kileng a lekoa ka botlalo 'me a netefatsoa hore ha a na li-endotoxins.Li-endotoxins ke lintho tse chefo tse tsoang maboteng a lisele tsa libaktheria tse itseng, 'me li ka baka litlamorao tse ngata tse mpe lits'ebetsong tsa bioloji, ho kenyelletsa ho ruruha le ts'ebetso ea ho itšireletsa mafung.Ka lehlakoreng le leng, metsi a ultrapure a bolela metsi a hloekisitsoeng ka tekanyo e phahameng ka ho fetisisa e ka khonehang, hangata ka mekhoa e kang reverse osmosis, deionization, le distillation, ho tlosa litšila tse kang li-ion, metsoako ea tlhaho le likaroloana.
E 'ngoe ea liphapang tsa bohlokoa lipakeng tsa metsi a se nang endotoxin le metsi a ultrapure ke lits'ebetsong tsa tsona tse fapaneng tsa tlhoekiso.Le ha metsi a ultrapure a ntse a etsa kalafo e matla ea 'mele le ea lik'hemik'hale ho tlosa litšila boemong ba limolek'hule, metsi a se nang endotoxin a shebane haholo le ho tlosoa ha li-endotoxin ka mekhoa e khethehileng ea tlhoekiso le tlhoekiso.Phapang ena e bohlokoa hobane le hoja li-endotoxin tse ling li ka tlosoa ka katleho ka mekhoa ea ho hloekisa metsi a mangata, ha ho na tiiso ea hore li-endotoxin tsohle li tla felisoa ntle le phekolo e khethehileng ea metsi e se nang endotoxin.
Phapang e 'ngoe ea bohlokoa lipakeng tsa mefuta e' meli ea metsi ke ts'ebeliso ea bona e reriloeng ho laboratoring le maemong a tlhahiso.Metsi a ultrapure a atisa ho sebelisoa lits'ebetsong moo ho ba sieo ha litšila boemong ba limolek'hule ho leng bohlokoa, joalo ka ho lokisoa ha li-reagents, li-buffers, le mecha ea litaba bakeng sa mekhoa ea lisele le liteko tsa baeloji ea limolek'hule.Ka lehlakoreng le leng, metsi a se nang endotoxin a etselitsoe ho sebelisoa litekong le lits'ebetsong moo boteng ba li-endotoxins bo ka senyang ho nepahala le ho tšepahala ha liphello.Sena se kenyelletsa lits'ebetso tse joalo ka lithuto tsa in vitro le in vivo, tlhahiso ea meriana, le tlhahiso ea lisebelisoa tsa bongaka, moo tšusumetso e ka bang teng ea li-endotoxins lits'ebetsong tsa cellular le tsa baeloji e tlamehang ho fokotsoa.
Ho bohlokoa ho hlokomela hore leha metsi a se nang endotoxin le metsi a ultrapure a sebeletsa merero e fapaneng, ha a khetholle.Ha e le hantle, libakeng tse ngata tsa laboratori le tsa tlhahiso, bafuputsi le bo-rasaense ba ka sebelisa mefuta e 'meli ea metsi ho itšetlehile ka litlhoko tse khethehileng tsa liteko le mekhoa ea bona.Ka mohlala, ha ho etsoa lisele ka laboratoring, metsi a ultrapure a ka sebelisoa bakeng sa ho lokisa mecha ea litaba ea lisele le li-reagents, ha metsi a se nang endotoxin a ka sebelisoa ho hlatsoa le ho lokisa libaka tsa lisele ho netefatsa hore ha ho na li-endotoxins tse ka kena-kenanang. liphetho tsa liteko.
Qetellong, ke habohlokoa ho hlokomela seometsi a se nang endotoxinle metsi a ultrapure ke mefuta e ikhethileng ea metsi a sebeletsang merero e fapaneng litsing tsa laboratori le tlhahiso.Ho utloisisa phapang pakeng tsa tse peli, ho kenyelletsa le mekhoa ea bona ea ho hloekisa le ho sebelisoa ho reretsoeng, ke habohlokoa bakeng sa ho netefatsa ho nepahala le ho tšepahala ha liphello tsa liteko.Ka ho sebelisa mofuta o loketseng oa metsi bakeng sa ts'ebeliso e 'ngoe le e' ngoe, bafuputsi le bo-ramahlale ba ka fokotsa kotsi ea tšilafalo le khopamo mosebetsing oa bona, 'me qetellong ba kenya letsoho tsoelo-peleng ea tsebo ea mahlale le boqapi.
Nako ea poso: Dec-06-2023
