Metsi a Reagent a LAL (Metsi a Teko ea Li-Endotoxins tsa Baktheria)

LAL Reagent Metsi(Metsi bakeng sa Teko ea Bacterial Endotoxins)

1. Tlhahisoleseding ea Sehlahisoa

LAL Reagent Metsi(Metsi bakeng sa Teko ea Li-Endotoxins tsa Baktheria kapa Metsi a BET kapa Metsi bakeng sa BET) ke metsi a se nang endotoxin a hloekisitsoeng ka ho khetheha a sebelisetsoa tlhahlobo ea Endotoxin.Khakanyo ea eona ea endotoxin e ka tlase ho 0.005 EU/ml.Liphutheloana tse fapaneng, tse kang 2ml, 10ml, 50ml, 100ml le 500ml yuniti ka 'ngoe, li fanoa molemong oa basebelisi.Metsi a Reagent a LAL (Metsi a BET) a ka sebelisoa ho hlapolla sampole ea tlhahlobo, ho aha sekhahla sa ho ema, kapa ho theha bocha li-reagents tsa Lyophilized Amebocyte Lysate.

2. Parameter ea sehlahisoa

Boemo ba Endotoxin: ≤0.005 EU/ml

Metsi bakeng sa Teko ea Bacterial Endotoxins ke metsi a sebetsitsoeng ka ho khetheha a sebelisetsoa ho lemoha endotoxin.Motsoako oa eona oa endotoxin o ka tlase ho 0.005EU/ml.Re boetse re fana kametsi bakeng sa BETe nang le boemo ba endotoxin bo ka tlase ho 0.001EU/ml bakeng sa assay sensitivity 0.001 ho 5EU/ml kinetic chromogenic assay.

3. Likarolo tsa Sehlahisoa le Tšebeliso

Metsi a mahala a Endotoxin (Metsi a BET, metsi a LAL Reagent, metsi a mahala a Endotoxin kapa metsi a BET) ke metsi a sebetsitsoeng ka ho khetheha a reretsoeng ho nchafatsa Lyophilized Amebocyte Lysate le Control Standard Endotoxin (CSE), le ho hlapolla lisampole le litekanyetso tsa taolo ts'ebetsong ea tlhahlobo ea endotoxin. .

Bakeng sa ho tsosolosoa ha Lyophilized Amebocyte Lysate kapa Amebocyte Lysate, ho hlapolloa ha lisampole tsa liteko le Control Standard Endotoxin, ho lokisa li-buffers tse sa lefelloeng tsa endotoxin, le kaho ea curve e tloaelehileng.500ml BET metsi a mantlha a sebetsa ka har'a li-endotoxin tse tsoang lisebelisoa tsa bongaka.

Boikutlo ba Lyophilized Amebocyte Lysate le potency ea Control Standard Endotoxin li lekoa khahlanong le USP Reference Standard Endotoxin.

Lisebelisoa tsa reagent tsa Lyophilized Amebocyte Lysate li tla le litaelo tsa sehlahisoa, Setifikeiti sa Analysis.

Metsi a BET (metsi a tlhahlobo ea baktheria endotoxin) ke eng?

Teko ea Endotoxin ka metsinge leng metsi a se nang endotoxin, haholoholo a sebelisetsoa tlhahlobo ea endotoxin ts'ebetsong ea ho kopanya le ho hlapolla.

Joalo ka, ho theha bocha reagent ea lyophilized lysate, laola endotoxin e tloaelehileng, le disampole tsa dilutions.e boetse e sebelisoa ha ho etsoa sampole le kalafo.

Metsi a Bioendo BET (Metsi a BET) a ka bitsoa metsi a reagent a TAL kapa metsi a reagent ea LAL.” LAL (lyophilized amebocyte lysate) reagent” .

Ho hlahisa ronaMetsi bakeng sa BETtharollo ea tlhahlobo ea endotoxin lisebelisoa tsa bongaka haholo-holo bakeng sa lisampole tse qoelisoang kapa tse qhibilihang.E le mohato oa bohlokoa oa ho netefatsa polokeho le ts'epahalo ea lisebelisoa tsa bongaka, sehlahisoa sa rona se etselitsoe ho tsamaisa mekhoa ea ho ntša lisampole ka ho fana ka metsi a mangata a LAL.Ka ho fana ka tharollo e sebetsang hantle le e sebetsang haholoanyane, ntho ea metsi ea BET e ikemiselitse ho nolofatsa mokhoa oa tlhahlobo ea endotoxin bakeng sa lisebelisoa tsa bongaka, e le ho boloka nako le lisebelisoa bakeng sa bareki ba rona.

Letoto la metsi a BET le etselitsoe ho rarolla mathata le mathata a tlhahlobo ea endotoxin bakeng sa lisebelisoa tsa bongaka.Ka tlhoko ea bongata bo boholo ba metsi a reagent ea LAL mekhoeng ea ho ntša lisampole, tharollo ea rona e loketse lilaboratori le baetsi ba lisebelisoa tsa bongaka ba batlang ho ntlafatsa lits'ebetso tsa bona tsa tlhahlobo.Ka ho fana ka molumo o mongata oaMetsi a ts'ebetso ea LAL, sehlahisoa sa rona se thusa ho etsa tlhahlobo e phethahetseng le e felletseng, ho netefatsa liphetho tse nepahetseng le khotso ea kelello ho bareki ba rona.

Ntle le melemo ea eona e sebetsang, letoto lena le boetse le ithorisa ka moralo o bonolo oa basebelisi le kopanyo e se nang moeli ho liprothokholo tse teng tsa liteko.Ka ho tsepamisa maikutlo ho bonolo le katleho, tharollo ea rona e ka kenyelletsoa habonolo ho phallo ea mosebetsi ea laboratori le mekhoa ea tlhahlobo.Ka ho ntlafatsa mekhoa ea ho ntša lisampole le ho fana ka metsi a mangata a LAL, metsi a matlafatsa bareki ba rona ho ntlafatsa boleng le ho tšepahala ha tlhahlobo ea endotoxin bakeng sa lisebelisoa tsa bongaka.Re na le ts'epo ea hore tharollo ea rona e ncha e tla ba le tšusumetso e kholo ts'ebetsong ea tlhahlobo ea endotoxin, 'me qetellong e tlatse letsoho polokehong le katlehong ea lisebelisoa tsa bongaka indastering ea tlhokomelo ea bophelo.