Quantitative Endotoxin Assay Tharollo Bakeng sa WFI kapa Dialysate
Dialysis (Mokgwa o Monyane wa Kinetic Chromogenic)
Lilemong tsa bo-1970, ketsahalo e sa thabiseng ea hemodialysis ea "pyrogenic reaction" e ile ea qhoma United States.Liphuputso tsa morao-rao li fumane hore dialysate e ne e silafalitsoe ke libaktheria tsa Gram-negative.Phetoho ea pyrogen e bakoang ke hemodialysis ke haholo-holo hore pyrogen e ka har'a dialyzer le metsi a dialysis a feta tekanyo, 'me karolo ea pyrogen maling a mokuli ea eketseha nakong ea ts'ebetso ea dialysis, e bakang letoto la matšoao.E le ho qoba litlamorao tse mpe tsa boleng ba metsi ho bakuli, ho hlophisoa ha dialysate, ho hlatsuoa ha dialysate, le metsi a sebelisetsoang dialyzer le liphaephe tsa mali kaofela li hloka tšebeliso ea metsi a hloekisitsoeng a sa hloekang, e leng metsi a dialysis.Boleng ba bohloeki ba metsi a dialysis bo amana le bophelo bo botle le boleng ba bophelo ba bakuli ba bangata ba nang le bothata ba ho hloleha ha renal.Ka hona, ho bohlokoa ho laola boleng ba metsi a amanang le dialysis.
Lysate reagent micro KC e sebelisang theknoloji e nyane ea kinetic chromogenic e hloka feela 25 μl ea sampole le 25 μl ea Lysate reagent bakeng sa tlhahlobo ka 'ngoe.E na le matla a matla a ho thibela ho kena-kenana le ho ba le kutloisiso ho fihlela ho 0.005EU/ml.E loketse haholo bakeng sa tlhahlobo ea endotoxin ea lihlahisoa tse amanang le dialysis.E na le sesebelisoa se ikhethileng sa "micro-detection" sa 96-well plate/slat le lysate test microorganism ELx808, e hlokomelang ts'ebetso ea micro-quantification kinetic chromogenic endotoxin, 'me sistimi e iphumanela le ho e sekaseka mohatong o le mong.
Lihlahisoa tse amanang ts'ebetsong ea tlhahlobo ea tlhahlobo ea micro kinetic chromogenic endotoxin:
MKC kit: MKC0505VS;MKC0505V;MKC0505AS;MKC0505A, MPMC96.
Endotoxin-free sampling bottle, Catalog number PA10, 10ml volume, tharollo e kholoanyane e tla fanoa.
Li-tubes tsa tlhahlobo tse se nang Endotoxin, Nomoro ea Catalog T107540 le T1310018
Li-microplates tse se nang Endotoxin (tse tlosoang / li sa tlosoe), Catalog number MPMC96, 8 strips.
Malebela a se nang Endotoxin (1000ul le 250ul), Catalog number PT25096 kapa PT100096
Laola Endotoxin e Tloaelehileng, Nomoro ea Catalog CSE10V
BET metsi, Catalog nomoro TRW50 kapa TRW100
Sebali sa Microplate: ELx808
Re fana ka lisebelisoa tsa tlhahlobo ea Bioendo KT endotoxin, ELx808, mixer ea vortex le likhoele tsa microplate tse se nang pyrogen bakeng sa tharollo ea tlhahlobo ea endotoxin bakeng sa WFI kapa Dialysate.
