Ke litšobotsi life tsakinetic turbidimetric endotoxin test assayho hlahloba li-endotoxins ka lisampole?
Thekinetic turbidimetric endotoxin test assayke mokhoa o sebelisoang ho etsa tlhahlobo ea li-endotoxin ka lisampole.
E na le likarolo tse 'maloa:
1. Tekanyo ea kinetic: Teko e ipapisitse le tekanyo ea kinetic ea liphetoho tsa turbidity tse bakoang ke karabelo lipakeng tsa endotoxins le reagent ea coagulation.Ka ho tsoela pele ho beha leihlo turbidity ha nako e ntse e feta, e lumella ho tsebahatsa khatello ea endotoxin sampoleng.
2. Kutloelo-bohloko e phahameng: Tekanyetso ea kinetic turbidimetric e hlokolosi haholo 'me e ka lemoha maemo a tlaase a endotoxin ka lisampole.E ka lekanya ka nepo likhakanyo tsa endotoxin, leha e le maemong a tlase haholo, e netefatsa hore e fumanoa le ho lekanya ka ts'epo.
3. Mefuta e mengata e fapaneng ea matla: Teko e na le mefuta e mengata e fapaneng ea matla, e bolelang hore e ka lekanya likhahla tsa endotoxin ho pholletsa le spectrum e pharaletseng.Sena se lumella tlhahlobo ea lisampole tse nang le maemo a fapaneng a endotoxins, ho tloha tlase haholo ho isa ho tse phahameng, ntle le tlhoko ea ho hlapolloa kapa ho tsepamisa maikutlo.
4. Liphetho tse potlakileng: The kinetic turbidimetric assay e fana ka liphello tse potlakileng ha li bapisoa le mokhoa o tloaelehileng oa gel clot lal assay.Hangata e na le nako e khuts'oane ea tlhahlobo, e nolofalletsang tlhahlobo e potlakileng le tlhahlobo ea lisampole.Hangata liphetho li ka fumanoa nakong ea kotara metsotso ho fihlela lihora tse 2, ho itšetlehile ka lisebelisoa tse khethehileng tsa tlhahlobo le lisebelisoa tse sebelisoang.
5. Boiketsetso le maemo: Teko e ka etsoa ho sebelisoa lisebelisoa tse ikemetseng, tse kang li-microplate readers kapa li-analyzer tse khethehileng tsa endotoxin.Sena se lumella tlhahlobo ea boemo bo phahameng le ho netefatsa litekanyo tse tsitsitseng le tse tloaelehileng, ho fokotsa liphoso tsa batho le ho eketsa bokhoni.
6. Ho lumellana le mefuta e sa tšoaneng ea mehlala: Tekanyetso ea kinetic turbidimetric e lumellana le mefuta e mengata ea mehlala, ho akarelletsa le meriana, lisebelisoa tsa bongaka, biologics, le mehlala ea metsi.Ke mokhoa o feto-fetohang o ka sebelisoang liindastering le lits'ebetsong tse fapaneng moo tlhahlobo ea endotoxin e hlokahalang.
Ka kakaretso, tlhahlobo ea kinetic turbidimetric endotoxin e fana ka mokhoa o hlokolosi, o potlakileng, hape o ts'epahalang oa ho bona le ho lekanya li-endotoxin lisampoleng, e e etsa sesebelisoa sa bohlokoa taolong ea boleng le lits'ebetsong tsa tlhahlobo ea polokeho.
Nako ea poso: Jun-05-2023
