EtEraser™ HP Endotoxin Removal Kit

EtEraser™ SE Endotoxin Removal Kit

1. Boitsebiso ba sehlahisoa

Re litsebi tsa tlhahlobo ea endotoxin le endotoxinremoval.Re fana ka litharollo tse felletseng tse akaretsang mefuta eohle ea taolo ea endotoxin, tlhahlobo le tlhahlobo ea endotoxin.Lihlahisoa tsa rona li kenyelletsa lisebelisoa tsa lipatlisiso tsa laboratorisesebelisoa sa ho tlosa endotoxins 'me e ka phahamisoa ho fihla ho litšiea tsa tlhahiso ea indasteri.Lipopolysaccharide (LPS) ke endotoxin ea baktheria hape ke karolo e kholo ea mabota a lisele tsa baktheria ea gram-negative.Recombinantprotein e tsoang ho E.colius hangata e na le boemo bo phahameng ba endotoxins.Ho tlosoa ha li-endotoxin tsena ho hlokahala haholo bakeng sa lits'ebetso tse tlase.

EtEraser HP Endotoxin Removal Kitli etselitsoe ho tlosa endotoxin contamination ho tsoa litharollong tse nang le metsi.Li-kits li na le li-resinbinds tsa ho tlosa endotoxin ho fokotsa maemo a endotoxin lisampoleng tsa protheine ka ≥99% ka nako e ka tlase ho lihora tse 2.

Setsi sena sa ho tlosa endotoxin se ka sebelisoa ho protheine, DNA/RNA, polysaccharide le lisampole tse ling tsa tlhaho.

2.Likarolo tsa sehlahisoa

◆ botsitso bo phahameng - ha bo ame mosebetsi oa boholo ba sampole ea likokoana-hloko

◆ ho hlaphoheloa ha protheine e phahameng - > 95% ea ho hlaphoheloa ha protheine bakeng sa disampole tsa protheine

◆ ts'ebetso e phahameng ea ho tlosa - tlosa> 99% endotoxin, boemo ba endotoxin sampoleng bo ka ba tlase ho 0.1 EU/mlafter endotoxin

◆ mefuta e mengata ea ts'ebeliso - e ka sebelisoa ho tlosoa ha endotoxin bakeng sa liprotheine, li-peptide, li-antibodies, liente, polysaccharide le mehlala e meng ea likokoana-hloko.

EtEraserHP High Efficient Efficient ea ho tlosa endotoxin e sebelisa immobilized affinity ligandmodified PMB ho tlama le ho tlosa endotoxin ho tsoa tharollong ea metsi.The modifiedPMB (polymyxin B) ligand ke ligand e phahameng e khethehileng e tlamang endotoxin.Thecolumn e na le sekhahla se phahameng haholo sa ho hlaphoheloa ha protheine ho feta 95%.Boemo ba Endotoxin sampoleng bo ka ba ka tlase ho 0.1 EU/ml kamora ts'ebetso ea ho tlosa endotoxin.

Kitin e kenyelletsa kholomo ea ho tlosa endotoxin e kentsoeng esale pele ea 1.5 ml, Equilibration Buffer, Regeneration Buffer le li-tube tsa pokello e se nang pyrogen le malebela.Kholomo e na le matla a holimo a tlamang a> 2, 000, 000 EU / ml.Sehlahisoa sena se ka sebelisoa hape makhetlo a mahlano haeba se nchafalitsoe hantle.Affinity resin e fumaneha inslurry 'me e ka eketseha ka mokhoa oa biopharmaceutical.