Teko ea Endotoxins ke Eng?

Teko ea Endotoxins ke Eng?

Li-endotoxins ke limolek'hule tsa hydrophobic tseo e leng karolo ea lipopolysaccharide complex tse etsang karolo e ngata ea lera la ka ntle la baktheria ea Gram-negative.Li lokolloa ha libaktheria li shoa 'me lera la tsona le ka ntle le senyeha.Li-endotoxins li nkoa e le tsona tse ka sehloohong tse tlatsetsang karabelong ea pyrogenic.'Me lihlahisoa tsa botsoali tse silafalitsoeng ke li-pyrogens li ka lebisa ho nts'etsopele ea feberu, ho kenngoa ha karabelo ea ho ruruha, ho tšoha, ho hlōleha ha setho le lefu ho batho.

Teko ea li-endotoxins ke teko ea ho bona kapa ho lekanya li-endotoxin ho tsoa ho baktheria ea Gram-negative.

Mebutla e hiriloe ho lemoha le ho lekanya li-endotoxins lihlahisoa tsa meriana qalong.Ho ea ka USP, RPT e kenyelletsa ho beha leihlo bakeng sa ho eketseha ha mocheso kapa feberu ka mor'a ente ea meriana ka mebutlanyana.Mme 21 CFR 610.13(b) e hloka tlhahlobo ya pyrogen ya mmutla bakeng sa dihlahiswa tse itseng tsa baeloji.

Lilemong tsa bo-1960, Fredrick Bang le Jack Levin ba fumane hore li-amebocyte tsa makhala a pere li tla koala boteng ba li-endotoxins.TheLimulus Amebocyte Lysate(kapa Tachypleus Amebocyte Lysate) e ile ea ntlafatsoa ka tsela e nepahetseng ho nka sebaka sa RPT e ngata.Ho USP, tlhahlobo ea LAL e bitsoa tlhahlobo ea bacterial endotoxin (BET).'Me BET e ka etsoa ka ho sebelisa mekhoa e 3: 1) mokhoa oa gel-clot;2) mokhoa oa turbidimetric;3) mokhoa oa chromogenic.Litlhoko tsa tlhahlobo ea LAL li na le pH e nepahetseng, matla a ionic, mocheso le nako ea ho incubation.

Ha e bapisoa le RPT, BET e potlakile ebile e sebetsa hantle.Leha ho le joalo, BET ha e khone ho nkela RPT sebaka ka botlalo.Hobane tlhahlobo ea LAL e kanna ea sitisoa ke lintlha mme ha e khone ho bona li-pyrogens tse seng endotoxin.