Li-endotoxins ke limolek'hule tse nyane tse tsoang ho hydrophobic lipopolysaccharides (LPS) tse tsoang ka har'a lisele tse kantle tsa baktheria ea gram-negative.Endotoxins e na le ketane ea mantlha ea polysaccharide, O-specific polysaccharide sidechain (O-antigen) le karolo ea lipid, Lipid A, e ikarabellang bakeng sa litlamorao tse chefo.Baktheria e tšolla endotoxin ka bongata ha lisele li shoa le ha li ntse li hola ka mafolofolo le ho arohana.Escherichia coli e le 'ngoe e na le limolek'hule tse ka bang limilione tse 2 tsa LPS sele e le 'ngoe.
Endotoxin e ka silafatsa li-labware habonolo, 'me boteng ba eona bo ka fana ka liteko tsa in vitro le tsa vivo.'Me bakeng sa lihlahisoa tsa batsoali, lihlahisoa tsa botsoali tse silafalitsoeng ke li-endotoxins tse kenyelletsang LPS li ka lebisa ho nts'etsopele ea feberu, ho kenya karabelo ea ho ruruha, ho tšoha, ho hlōleha ha setho le lefu ho motho.Bakeng sa lihlahisoa tsa dialysis, LPS e ka fetisetsoa ka membrane e nang le boholo bo boholo ba pore ka ho hloekisa ka morao ho tloha ho dialysis fluid ho ea maling, mathata a ho ruruha a ka bakoa ka tsela e nepahetseng.
Endotoxin e fumanoa ke Lyophilized Amebocyte Lysate (TAL).Bioendo e nehetsoe ho etsa lipatlisiso, ho nts'etsapele le ho hlahisa reagent ea TAL ka lilemo tse fetang mashome a mane.Lihlahisoa tsa rona li akaretsa mekhoa eohle e sebelisoang ho bona endotoxin, e leng mokhoa oa gel-clot, mokhoa oa turbidimetric, le mokhoa oa chromogenic.
Nako ea poso: Jan-29-2019
