Lisebelisoa tse se nang pyrogen ke lintho tse ka sebelisoang ntle le endotoxin e tsoang kantle, ho kenyeletsoa malebela a pipette a se nang pyrogen (lebokose la ntlha), li-tubes tsa tlhahlobo tse se nang pyrogen kapa tse bitsoang li-endotoxin mahala tsa khalase, li-ampoules tsa khalase tse se nang pyrogen, li-microplates tse se nang endotoxin tse 96, le endotoxin- metsi a sa lefelloeng (tšebeliso ea metsi a depyrogenated tekong ea baktheria ea endotoxin), endotoxin mahala buffer le joalo-joalo Har'a tsona, metsi bakeng sa baktheria endotoxintest ka mokhoa oa gel clot le tekanyo ea tlhahlobo ea tlhahlobo ea endotoxin likhatisong tsohle tse amanang le Pharmacopoeia (USP, EP, BP, JP le China Pharmaceutica) .E bua ka metsi a hloekisitsoeng bakeng sa ente e nang le endotoxin e ka tlase ho 0.015EU/ml.Hona joale mofuta oa morao-rao oa Pharmacopoeia, metsi a BET a ka tlase ho 0.005EU/ml.Esita le boemo bo phahameng ka ho fetisisa bo ka tlaase ho 0.001EU/ml bo khona ho hlahisoa le ho fanoa ke Bioendo.
Mantsoe a bohlokoa a amanang le li-endotoxins, le lisebelisoa tsa mahala tsa endotoxin, mehloli ea pyrogen le mocheso ke likhopolo tse peli tse fapaneng ka ho feletseng:Pyrogen: E boetse e bitsoa pyrogen kapa exothermic factor.dintho tse ka bakang keketseho ya mocheso wa mmele.Mohloli oa Mocheso: Ntho e ntshang mocheso.Joalo ka mecheso e tukang, mashala, joalo-joalo.Seo ho thoeng ke "lisebelisoa tse seng tsa pyrogenic" le "pyrogenic response" tsa baetsi ba bang le bahoebi ha e le hantle ke mabitso a sa tsitsang le a khelosang haholo.Tse nepahetseng li lokela ho ba "Pyrogen Free" le "Pyrogen Response".
Hobaneng ha lisebelisoa tsa mahala tsa Pyrogen li hlokahala tekong ea tlhahlobo ea endotoxin, tlhahlobo ea tlhahlobo ea gel clot endotoxin le tlhahlobo ea tlhahlobo ea endotoxin?
Ee, lisebelisoa tse se nang pyrogen li bohlokoa bakeng sa ho etsa tlhahlobo ea endotoxin ka nepo le ka ts'epo.Ho ba teng ha li-pyrogens, e leng lintho tse bakang feberu hangata tse tsoang ho li-endotoxin tsa baktheria, li ka kena-kenana le liphello tsa tlhahlobo 'me tsa lebisa ho baleng ba bohata.Teko ea endotoxin, eo hangata e tsejoang ka hore ke tlhahlobo ea Limulus amebocyte lysate (LAL) kapa e bitsoang Lyophilized amebocyte lysate (LAL), e sebelisetsoa ho bona le ho lekanya boteng ba li-endotoxin tsa baktheria litlhare, lisebelisoa tsa bongaka le lihlahisoa tse ling.Teko ea LAL e ipapisitse le karabelo lipakeng tsa LAL reagent le endotoxins ho hlahisa karabelo e koalang kapa ea chromogenic.Ho netefatsa liphetho tse nepahetseng, ho bohlokoa ho sebelisa lisebelisoa tse se nang pyrogens.Li-pyrogens li ka silafatsa lisebelisoa tse fapaneng tsa laboratori, ho kenyeletsoaglassware, malebela a pipette, li-tubes, le lijana tsa mohlala.Haeba lisebelisoa tse silafalitsoeng ke pyrogen li ka kopana le reagent ea LAL kapa lisampole tsa liteko, li ka tsosa maikutlo a fosahetseng, a lebisang ho liqeto tse fosahetseng mabapi le boteng kapa bongata ba li-endotoxins.Lisebelisoa tse se nang pyrogen li etsoa le ho lekoa ka ho khetheha ho fokotsa kapa ho felisa boteng ba li-pyrogens.Ba kena lits'ebetsong tse matla tsa taolo ea boleng ho netefatsa hore ba fihlela litekanyetso tse hlokahalang bakeng sa tlhahlobo ea endotoxin.Ho sebelisa lisebelisoa tsena tse khethehileng ho thusa ho boloka botšepehi le ho nepahala ha tlhahlobo ea tlhahlobo ea endotoxin, ho netefatsa liphetho tse tšepahalang le ho khothaletsa polokeho ea mokuli lits'ebetsong tsa meriana le tsa biomedical.
Nako ea poso: Nov-07-2022
