Teko ea Recombinant Factor C (rFC).ke mokhoa o sebelisoang haholo oa ho lemoha li-endotoxins tsa baktheria, tse tsejoang hape e le lipopolysaccharides (LPS), Endotoxins ke karolo ea lera le ka ntle la libaktheria tsa Gram-negative tse ka bakang karabelo e matla ea ho ruruha ho liphoofolo, ho akarelletsa le batho.Tekolo ea rFC e ipapisitse le tšebeliso ea mofuta o entsoeng ka liphatsa tsa lefutso oa Factor C, enzyme eo ka tlhaho e fumanoang maling a makhala a pere 'me e ameha tseleng ea ho hoama.Tekong ea rFC, recombinant Factor C e sebelisetsoa ho bona boteng ba li-endotoxins ka ho lekanya Ka ho lekanya litaba tsa substrates tse petsotsoeng ka boteng ba endotoxin.Ha ho bapisoa le mekhoa ea khale ea ho lemoha endotoxin, joalo ka tlhahlobo ea Limulus Amebocyte Lysate (LAL) e sebelisang mali a makhala a pere, tlhahlobo ea rFC e nkoa e le ea maemo a holimo le e ka hlahisoang hape, kaha ha e itšetlehe ka tšebeliso ea li-reagents tse entsoeng ke liphoofolo.Tekolo ea rFC e boetse e na le botsoalle haholoanyane tikolohong le ho tšoarella, kaha e fokotsa tlhoko ea pokello le ts'ebeliso ea makhala a lipere ho lemoha endotoxin.
Teko ea rFC e amohetsoe ke balaoli ba taolo, joalo ka United States Pharmacopeia (USP) , European Pharmacopoeia (EP) le Chinese Pharmacopoeia (CP) hore e sebelisoe tlhahlobong ea taolo ea boleng ba meriana le lisebelisoa tsa bongaka.
Melemo ea recombinant factor c assay
Teko ea Recombinant Factor C (rFC) e fana ka melemo e mengata ho feta mekhoa ea setso ea ho bona li-endotoxins, joalo ka tlhahlobo ea Limulus Amebocyte Lysate (LAL).Melemo e meng ea rFC assay e kenyelletsa:
1. Maemo: Tekolo ea rFC ke theknoloji e kopanetsoeng ea DNA e sebelisang protheine e le 'ngoe, e hlalosoang e le sesebelisoa sa ho lemoha.Sena se etsa hore tlhahlobo e be e tloaelehileng le e fokolang haholo ha e bapisoa le tlhahlobo ea LAL, e itšetlehileng ka tšebeliso ea motsoako o rarahaneng oa liprotheine tse nkiloeng maling a makhala a pere.
2. Reproducibility: Tekanyetso ea rFC e na le maemo a phahameng a ho ikatisa, kaha e sebelisa protheine e le 'ngoe, e hlalositsoeng e le reagent ea ho lemoha.Sena se lumella liphetho tse tsitsitseng, esita le ka har'a lihlopha tse fapaneng le li-reagents tse ngata.
3. Tšebeliso e fokolitsoeng ea liphoofolo: Tekolo ea rFC ke mokhoa o baballang tikoloho le o tsitsitseng haholoanyane oa ho lemoha li-endotoxins, kaha ha o hloke tšebeliso ea liphoofolo tse phelang kapa tse entsoeng sehlabelo, tse kang makhala a lipere.
4. Ho boloka litšenyehelo: Tekanyo ea rFC ka kakaretso e boloka chelete e ngata ho feta teko ea LAL, ka lebaka la tlhokahalo e fokotsehileng ea liphoofolo tse phelang le mokhoa o tloaelehileng oa tlhahlobo.
5. Botsitso: Tlhahlobo ea rFC e matla 'me e ka sebelisoa mefuteng e mengata ea likopo, ho kenyelletsa le tlhahlobo ea taolo ea boleng ba meriana, lisebelisoa tsa bongaka, le lihlahisoa tse ling tse ka' nang tsa e-na le li-endotoxins.
6. Tumello ea taolo: Tekanyetso ea rFC e amohetsoe ke balaoli ba taolo joalo ka United States Pharmacopeia (USP) , European Pharmacopoeia (EP) le Chinese Pharmacopoeia (CP) bakeng sa ho sebelisoa tekong ea taolo ea boleng ba meriana le lisebelisoa tsa bongaka.Sena se fana ka boemo bo phahameng ba kholiseho ea ho tšepahala le ho nepahala ha tlhahlobo.
Ho fihlela litlhoko tse fapaneng, Bioendo e boetse e hlahisa le ho fana ka mokhoa oa setso oa gel clot endotoxin test assay kit, quick gel clot assay kit, quantitative endotoxin test assay kit ho kenyeletsa "kinetic turbidimetric endotoxin test assay kitlekinetic chromogenic endotoxin test assay kit” .
Nako ea poso: Feb-19-2023
