Metsi a se Nang Endotoxin: Ho bapala Karolo ea Bohlokoa Litlhahlobong tsa Tlhahlobo ea Endotoxin
Selelekela:
Teko ea Endotoxin ke karolo ea bohlokoa ea liindasteri tse fapaneng, ho kenyeletsoa meriana, lisebelisoa tsa bongaka le biotechnology.Ho lemoha ka nepo le ho ts'epahala ha li-endotoxins ho bohlokoa ho netefatsa polokeho ea sehlahisoa le ho latela litekanyetso tsa taolo.Tlhokahalo e 'ngoe ea mantlha ea ho etsa tlhahlobo ea endotoxin ke tšebeliso ea metsi a se nang endotoxin.Sehloohong sena, re tla hlahloba bohlokoa ba metsi a se nang endotoxin, karolo ea eona ho etseng liteko tsa Lyophilized Amebocyte Lysate (LAL) endotoxin, le bohlokoa ba ho sebelisa metsi a se nang endotoxin ho Bacterial Endotoxin Test (BET).
Ho utloisisa Endotoxins:
Li-endotoxins ke lipopolysaccharides (LPS) tse fumanehang lera le ka ntle la baktheria ea Gram-negative.Ke bakena-lipakeng ba matla ba ho ruruha 'me ba ka baka liphello tse mpe haholo ha li le teng lihlahisoa tsa meriana kapa lisebelisoa tsa bongaka.Ka lebaka la bokhoni ba bona ba ho baka karabelo ea pyrogenic, ho lemoha ka nepo le ho lekanya palo ea li-endotoxin ho bohlokoa.
Teko ea LAL Endotoxin:
Mokhoa o tsebahalang haholo oa tlhahlobo ea endotoxin ke tlhahlobo ea LAL, e sebelisang mali a makhala a pere.Limulus polyphemus le Tachypleus tridentatus.Reagent ea Lyophilized Amebocyte Lysate (LAL) e ntšoa liseleng tsa mali tsa makhala ana, tse nang le protheine e koalang e kentsoeng ka pel'a li-endotoxins.
Karolo eaMetsi a se Nang Endotoxinlitekong tsa LAL:
Metsi ke karolo ea mantlha methating ea tokiso ea reagent le mehato ea ho hlapolla tlhahlobo ea LAL.Leha ho le joalo, esita le ho latela palo ea li-endotoxin tse teng metsing a pompo a tloaelehileng li ka kena-kenana le ho nepahala le kutloisiso ea tlhahlobo.Ho hlola phephetso ena, metsi a se nang endotoxin a tlameha ho sebelisoa nakong eohle ea tlhahlobo.
Metsi a se nang Endotoxin a bapala karolo ea bohlokoa ho netefatsa hore li-reagents tse sebelisoang tlhahlobong ea LAL ha li silafatsoe ke li-endotoxin.Ho feta moo, e thibela liphetho tse fosahetseng kapa tse fosahetseng, ka hona e fana ka palo e tšepahalang le e nepahetseng ea endotoxin.
Ho Khetha Metsi a Nepahetseng bakeng sa Tlhahlobo ea LAL:
Ho fumana metsi a se nang endotoxin, ho ka sebelisoa mekhoa e mengata ea tlhoekiso.Deionization, distillation, le reverse osmosis ke mekhoa e sebelisoang hangata ho fokotsa boteng ba endotoxins metsing.Mekhoa ena e tlosa litšila tse fapaneng, ho kenyelletsa le li-endotoxin tse tsoang ho baktheria.
Ho feta moo, ho bohlokoa ho etsa bonnete ba hore lijana tse sebelisetsoang ho boloka, ho bokella le ho aba metsi a se nang endotoxin li netefalitsoe ka nepo ebile ha li na tšoaetso ea endotoxin.Sena se kenyelletsa ho sebelisa li-tubes, libotlolo le li-filters tse se nang endotoxin nakong ea ts'ebetso.
Bohlokoa ba Metsi a BET:
HoTeko ea Baktheria Endotoxin (BET), metsi a se nang endotoxin, a tsejoang hape e le metsi a BET, a sebelisoa e le taolo e mpe ho netefatsa kutlo le ho hlaka ha tlhahlobo ea LAL.Metsi a BET a lokela ho ba le boemo bo sa bonahaleng ba li-endotoxin, ho netefatsa hore ts'ebetso efe kapa efe e ka lekanngoang ea endotoxin e nkiloe feela sampoleng e lekiloeng.
Tšebeliso ea metsi a BET tekong ea endotoxin e sebetsa e le taolo ea bohlokoa ho netefatsa katleho ea li-reagents tsa LAL, sistimi ea liteko le lisebelisoa.Mohato ona oa netefatso o bohlokoa ho lekola boteng le khatello ea li-endotoxin sampoleng e lekiloeng.
Qetello:
Metsi a se nang Endotoxin a bapala karolo ea bohlokoa ho fumanoeng ka nepo le ka ts'epahalo ea li-endotoxin liindastering tse fapaneng.Tekong ea LAL endotoxin, e netefatsa hore li-reagents tse sebelisitsoeng ha li na tšoaetso, e fana ka palo e nepahetseng.Ho BET, metsi a se nang endotoxin a sebetsa e le taolo, a netefatsa kutloisiso ea tlhahlobo ea LAL.Ka ho latela mekhoa e thata ea tlhoekiso le ho sebelisa lijana tse netefalitsoeng, monyetla oa liphetho le liphoso tse fosahetseng li ka fokotsoa haholo.
Ha bohlokoa ba tlhahlobo ea endotoxin bo ntse bo hola, karolo ea metsi a se nang endotoxin e ba ea bohlokoa le ho feta.Ho sebelisa mekhoa e tšepahalang ea ho hloekisa metsi le ho kenyelletsa mekhoa e metle ts'ebetsong ea tlhahlobo ho tla netefatsa polokeho le ho lateloa ha lihlahisoa tsa meriana, lisebelisoa tsa bongaka le lisebelisoa tse ling tse sa utloeng bohloko ke endotoxin.
Nako ea poso: Nov-29-2023
