Limulus amebocyte lysate (LAL) kapa Tachypleus tridentatus lysate (TAL) ke mokelikeli o nang le metsi a tsoang ho makhala oa pere.
'Me li-endotoxins ke limolek'hule tsa hydrophobic tseo e leng karolo ea lipopolysaccharide complex tse etsang karolo e ngata ea lera la ka ntle la libaktheria tsa Gram-negative.Lihlahisoa tsa batsoali tse silafalitsoeng ke li-pyrogens li ka lebisa liphellong tse tebileng tse kang feberu, ho tšoha, ho hlōleha ha setho sa 'mele, esita le lefu.
LAL/TAL reagent e ka sebetsana le endotoxin ea baktheria le lipopolysaccharide (LPS).Bokhoni ba ho tlama le ho koala endotoxin ea LAL ke eona e e etsang hore e be ea bohlokoa haholo indastering ea rona ea meriana.Ke ka lebaka leo reagent ea LAL/TAL e ka sebelisoang ho bona kapa ho lekanya endotoxin ea baktheria.
Pele ho fumanoa hore LAL/TAL e ka sebelisoa ho etsa tlhahlobo ea baktheria endotoxins, meutlanyana e sebelisoa ho bona le ho lekanya li-endotoxins lihlahisoa tsa meriana.Ha ho bapisoa le RPT, BET e nang le reagent ea LAL/TAL e potlakile ebile e sebetsa hantle, 'me ke mokhoa o tsebahalang oa ho etsa tlhahlobo e matla ea khatello ea endotoxin indastering ea meriana, joalo-joalo.
Teko ea tlhahlobo ea gel clot endotoxin, e tsejoang hape e le tlhahlobo ea Limulus Amebocyte Lysate (LAL), kapa e bitsoang Lyophilized Amebocyte Lysate (LAL) ke mokhoa o sebelisoang haholo ho lemoha le ho lekanya li-endotoxin lihlahisoa tse fapaneng, haholo indastering ea meriana le lisebelisoa tsa bongaka.E nkoa e le tharollo e hlokahalang tšimong ea ho lemoha endotoxin ka lebaka la katleho ea eona le ho amoheloa ka molao.
Tlhahlobo ea LAL e itšetlehile ka molao-motheo oa hore lisele tsa mali tsa makhala a lipere (Limulus polyphemus kapa Tachypleus tridentatus) li na le ntho e thibelang mali e sebetsanang le li-endotoxin tsa baktheria, e leng se etsang hore ho thehoe lesela le kang la gel.Karabelo ena e na le kutloelo-bohloko haholo 'me e tobane le li-endotoxins, e leng likarolo tse chefo tsa lera le ka ntle la baktheria ea gram-negative.
Ho na le mabaka a 'maloa a etsang hore tlhahlobo ea tlhahlobo ea gel clot endotoxin e nkoe e le tharollo e hlokahalang bakeng sa ho lemoha endotoxin:
1. Kamohelo ea Taolo: Teko ea LAL e amoheloa le ho amoheloa ke balaoli ba taolo ba kang United States Pharmacopeia (USP) le European Pharmacopoeia (EP) e le mokhoa o tloaelehileng oa tlhahlobo ea endotoxin.Ho latela melao ena ke tlamo bakeng sa ho netefatsa polokeho le boleng ba lihlahisoa tsa meriana.
2. Sensitivity and Specificity: Tlhahlobo ea LAL e na le kutloisiso e phahameng, e lumellang ho lemoha maemo a tlaase haholo a endotoxins.E khona ho bona likhahla tsa endotoxin tse tlase ho 0.01 endotoxin units ka millilithara (EU/mL).Ho nepahala ha tlhahlobo ho netefatsa hore e lemoha li-endotoxins le ho fokotsa liphetho tse fosahetseng.
3. Katleho ea Litšenyehelo: Tlhahlobo ea tlhahlobo ea gel clot endotoxin ka kakaretso e nkoa e le tharollo ea moruo ha e bapisoa le mekhoa e meng e kang ea chromogenic kapa turbidimetric assays.E hloka li-reagents le lisebelisoa tse fokolang, ho fokotsa litšenyehelo tsa tlhahlobo ka kakaretso.Ntle le moo, ho fumaneha ha li-reagents tse emeng tsa LAL 'marakeng ho etsa hore ho be bonolo hore li laboratori li etse tlhahlobo.
4. Maemo a Indasteri: Teko ea LAL e amohetsoe haholo indastering ea meriana le lisebelisoa tsa bongaka e le mokhoa o tloaelehileng oa ho lemoha endotoxin.Ke karolo ea bohlokoa ea mekhoa ea ho laola boleng nakong ea tlhahiso ea lihlahisoa tsa meriana le lisebelisoa tsa bongaka, ho netefatsa hore ho latela litlhoko tsa taolo.
Leha ho le joalo, ke habohlokoa ho hlokomela hore tlhahlobo ea tlhahlobo ea gel clot endotoxin e ka 'na ea e-ba le mefokolo, e kang ho kena-kenana le lintho tse itseng le monyetla oa liphello tse fosahetseng kapa tse fosahetseng.Maemong a ikhethileng, mekhoa e meng e joalo ka liteko tsa chromogenic kapa turbidimetric e ka sebelisoa ho tlatselletsa kapa ho netefatsa liphetho tse fumanoeng tlhahlobong ea LAL.
Nako ea poso: Apr-29-2019
