Endotoxin Test Assay ka Lyophilized Amebocyte Lysate (LAL Reagent)

Endotoxin Test Assay ka Lyophilized Amebocyte Lysate (LAL Reagent)

Li-reagents tsa LAL: Lyophilized amebocyte lysate (LAL) ke motsoako o nang le metsi a lisele tsa mali (amebocyte) ho tloha makhala a Atlantic horseshoe.
Li-reagents tsa TAL: Reagent ea TAL ke karolo ea lisele tsa mali tse tsoang ho Tachypleus tridentatus.
Hajoale, tlhahiso ea mantlha ea li-reagents tsa LAL/TAL li United States le China.

Gel clot endotoxin test assay, mokhoa ona e ntse e le ts'ebeliso ea mantlha lefats'eng lohle, molemong oa ho fihlela tlhoko ea polokeho ea meriana ea ente ea Motho.
HONA joale Bioendo e hlahisa le ho fana ka gel clot LAL reagent ho kenyelletsa le li-ampule tsa khalase e le 'ngoe le linkho tse ngata tsa tlhahlobo.
https://www.bioendo.com/gel-clot-endotoxin-assay/ G01, GS44, G02, G17 le G52
Ena ke tharollo ea moruo bakeng sa ho lemoha li-endotoxins tsa boleng tekong ea lithethefatsi.Haholo-holo bakeng sa lithethefatsi tse nang le ente kapa lithethefatsi tsa batsoali ho hlahloba li-endotoxins ho WFI, API kapa lihlahisoa tsa lithethefatsi tse felileng.Teko ea tlhahlobo ea Endotoxin ke tlhoko e phahameng ea ts'ebetso, e hloka opereishene e hloahloa ho sebetsana le tlhahlobo ea leqeba la gel ho netefatsa hore ho na le liphetho tse nepahetseng.

Tharollo e feletseng bakeng saG52Tlhahlobo ea Endotoxin:
LAL Reagent
Laola Endotoxin e Tloaelehileng
BET Metsi
Malebela a pipette a se nang Endotoxin
Li-tubes tsa khalase tse se nang endotoxin, ho kenyelletsa ts'ebetso ea dilution le li-tubes reaction.
Sesebelisoa sa ho qhoqhoa, ho khothaletsa bate ea metsi kapa mochine o omileng oa mocheso.Lisebelisoa tsohle tsa incubation li hloka ho nepahala ha mocheso.
Lintho tsohle tse sebelisoang ho ama tlhahlobo ea LAL e kopanang le maemo a se nang endotoxin ” <0.005EU/ml ” .
Tikoloho ea liteko e tla lumellana le tlhahlobo ea endotoxin.

Ela hloko :
Lisebelisoa tsa polasetiki tse kang malebela a pipette kapa lipoleiti tsa multiwell li sebelisoa haholo bakeng sa tlhahlobo ea endotoxin.The harmonized Pharmacopoeias (USP/CP) e hloka hore lisebelisoa life kapa life tsa polasetiki le li-tubes tsa khalase li tlameha ho se be le endotoxin e bonoang hape e le tsa bohlokoa ka ho ts'oana empa hangata li sa nkuoe, li tlameha ho ba maemo a se nang mabaka a sitisang.

Mokhoa oa ho sebelisa tlhahlobo ea endotoxin ho bona li-endotoxins sampuling?
Pele ho tsohle, tlhahlobo ea netefatso ea sensitivity ea lysate e lokela ho etsoa.Ho netefatsa kutloisiso e ts'oana le matshwao a leibole.
Bakeng sa tlhahlobo ea sampole, etsa liteko tsa tlhahlobo ea endotoxin pele ho tšitiso.
Ho sebelisa tlhahlobo e felletseng ea endotoxin ea "teko ea tšitiso".
Ho etsa tlhahlobo ea moeli bakeng sa ho fumana hore na boemo ba endotoxins bo joang sampoleng.

Ha o sebetsa tlhahlobo ea netefatso ea "lysate sensitivity" e ngotsoeng, sephetho ke se sa tloaelehang, 2 Lamada havenot gel formation?
Lekola hore na mokhoa oa ho lokisa oa endotoxin e tloaelehileng ea taolo ea baktheria o nepahetse.
Ho kopanngoa ha Vortex hoa hlokahala bakeng sa hlapollo e 'ngoe le e 'ngoe (sheba bukana ea litaelo kapa e bitsoang ho kenya endotoxin e tloaelehileng ea taolo ka botlalo).
Ho hlahloba hore na reagent ea lysate e lumellana le endotoxin e tloaelehileng ea taolo.

Ho kgothaletswa hore o sebelise ho hlapa metsi kapa mocheso o omileng oa mocheso bakeng sa ts'ebetso ea mocheso e sa khaotseng ea reagent ea lysate (mohlala, incubator kapa lebokose le omeletseng ha le sebetse).
Nakong ea incubation, tikoloho ea metsi ka bateng ea metsi e tsitsitseng le e tsitsitseng, ho thothomela hohle hoa qojoa.
Ela hloko ka ho khetheha ho hlapa metsi ho tima pompo ea phallo ea metsi.

Ha u ahlola sephetho, ka bonolo ntša tube ea teko kapa ampoule ea khalase ho thermostat, butle-butle fetola likhato tse 180,
sebopeho sa gel ka har'a tube se ke ke sa senyeha, 'me se ke ke sa theoha, sephetho se tlalehiloe ka letšoao "+";
ha ho sebopeho sa gel se entsoeng kapa lesela la gel le ke ke la bolokoa le tiile le hoja le entsoe.
Sekhahla sa lebota se fosahetse, sephetho se tlalehiloe ka lets'oao "-".
Potlako ea gel clot endotoxin test Kit ke ea mokhoa oa tlhahlobo ea gel clot endotoxin.
Tekong e potlakileng ea gel clot endotoxin, mohlala oa taolo e ntle ha o hlahise gel?
Ntlha ea pele, sebelisa metsi a tlhahlobo ea baktheria endotoxin ho netefatsa li-reagents tsa lysate, ho netefatsa hore basebetsi le tikoloho ba kopane le melao;

Haeba kit e tšoaneleha, joale taolo e ntle ea sampuli ha e na sebopeho sa gel ka lebaka la phello e thibelang ea sampuli, 'me sampuli e lokela ho sebetsoa le ho phekoloa.
Mokhoa o atileng haholo oa ho sebetsa sampole ke dilution.