KCET- Kinetic Chromogenic Endotoxin Test Assay (Teko ea Chromogenic endotoxin ke mokhoa oa bohlokoa oa lisampole tse nang le tšitiso e itseng.)
Teko ea kinetic chromogenic endotoxin (KCT kapa KCET) ke mokhoa o sebelisoang ho bona boteng ba endotoxin sampuling.
Li-endotoxins ke lintho tse chefo tse fumanehang maboteng a lisele tsa mefuta e itseng ea libaktheria, ho kenyeletsoa libaktheria tsa gram-negative joalo ka Escherichia coli le Salmonella.Tekong ea KCET, substrate ea chromogenic e eketsoa sampoleng, e sebetsanang le li-endotoxin tse teng ho hlahisa phetoho ea 'mala.
Sekhahla sa nts'etsopele ea mebala se beoa leihlo ha nako e ntse e ea ho sebelisoa spectrophotometer, 'me palo ea endotoxin sampuling e baloa ho ipapisitsoe le sekhahla sena.
Teko ea KCT ke mokhoa o tsebahalang oa ho bona li-endotoxins litlhare, lisebelisoa tsa bongaka le lihlahisoa tse ling tse kopanang le 'mele oa motho.Ke teko e hlokolosi le e ka tšeptjoang e ka bonang esita le palo e nyenyane haholo ea endotoxin, e leng se etsang hore e be sesebelisoa sa bohlokoa ho netefatsa polokeho ea lihlahisoa tsena.
TAL/LAL reagent ke lyophilized amebocyte lysate e ntšitsoeng maling a maputsoa a Limulus polyphemus kapa Tachypleus tridentatus.
Endotoxins ke amphiphilic lipopolysaccharides (LPS) e fumanehang ka har'a lera la lisele tsa kantle tsa baktheria ea gram-negative.Lihlahisoa tsa batsoali tse silafalitsoeng ke li-pyrogens tse kenyeletsang LPS li ka lebisa ho nts'etsopele ea feberu, ho kenya karabelo ea ho ruruha, ho tšoha, ho hlōleha ha setho le lefu ho motho.
Ka hona, linaha ho pota lefatše li thehile melaoana, e hlokang hore sehlahisoa leha e le sefe sa lithethefatsi se ipolelang hore se nyopa ebile ha se pyrogenic se lokela ho hlahlojoa pele se lokolloa.Teko ea TAL ea li-gel-clot e ile ea etsoa pele bakeng sa tlhahlobo ea li-endotoxins tsa baktheria (ke hore, BET).
Leha ho le joalo, mekhoa e meng e tsoetseng pele ea tlhahlobo ea TAL e hlahile.'Me mekhoa ena e ke ke ea lemoha feela empa hape e tla lekanya boteng ba li-endotoxin ka sampuling.Ntle le mokhoa oa gel-clot, mekhoa ea BET e boetse e na le mokhoa oa turbidimetric le mokhoa oa chromogenic.Bioendo, e ikemiselitseng ho lemoha endotoxin, ke moetsi ea hloahloa oa ho etsa tlhahlobo ea TAL/LAL ea chromogenic.
Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay) e fana ka tekanyo e potlakileng ea tekanyo ea endotoxin.
Re boetse re fana ka Bioendo KC Endotoxin Test Kit (Kinetic Chromogenic Assay) le incubation microplate reader ELx808IU-SN, e ka netefatsang ho tšepahala le ho sebetsa hantle ha liteko tsa hau.
Ke litšobotsi life tsatlhahlobo ea kinetic chromogenic endotoxinho hlahloba li-endotoxins ka lisampole?
Teko ea teko ea kinetic chromogenic endotoxin ke mokhoa o mong o sebelisoang ho lekola li-endotoxin lisampoleng.E na le likarolo tse 'maloa:
1. Tekanyo ea kinetic: Ho tšoana le tlhahlobo ea turbidimetric, kinetic chromogenic assay e boetse e kenyelletsa tekanyo ea kinetic.E itšetlehile ka karabelo pakeng tsa endotoxins le substrate ea chromogenic ho hlahisa sehlahisoa se mebala.Phetoho ea matla a 'mala ha nako e ntse e ea e behiloe leihlo, e leng se lumellang hore ho be le palo ea likhakanyo tsa endotoxin sampoleng.
2. Kutloelo-bohloko e phahameng: Tekanyetso ea kinetic chromogenic e hlokolosi haholo 'me e ka lemoha maemo a tlaase a endotoxin ka lisampole.E khona ho lekanya ka nepo likhakanyo tsa endotoxin, leha e le maemong a tlase haholo, e netefatsa hore e fumaneha ka nepo le ho lekanya palo.
3. Mefuta e mengata e fapaneng ea matla: Teko e na le mefuta e mengata e fapaneng, e lumellang ho lekanya likhahla tsa endotoxin ho pholletsa le spectrum e pharaletseng.Sena se bolela hore e ka leka lisampole ka maemo a fapaneng a li-endotoxins, tse amohelang likhakanyo tse tlase le tse phahameng ntle le tlhoko ea ho hlapolloa kapa ho tsepamisa maikutlo.
4. Liphetho tse potlakileng: Teko ea kinetic chromogenic e fana ka liphello tse potlakileng ha li bapisoa le mekhoa e tloaelehileng.Hangata e na le nako e khuts'oane ea tlhahlobo, e nolofalletsang tlhahlobo e potlakileng le tlhahlobo ea lisampole.Tsoelo-pele ea mebala e ka hlahlojoa ka nako ea sebele, 'me hangata liphetho li ka fumanoa ka mor'a metsotso e seng mekae ho ea ho lihora tse' maloa, ho itšetlehile ka lisebelisoa tse khethehileng tsa tlhahlobo le lisebelisoa tse sebelisoang.
5. Boiketsetso le maemo: Teko e ka etsoa ho sebelisoa lisebelisoa tse ikemetseng, joalo ka li-microplate readers kapa.
li-analyzers tse khethehileng tsa endotoxin.Sena se lumella tlhahlobo ea boemo bo phahameng le ho netefatsa litekanyo tse tsitsitseng le tse tloaelehileng, ho fokotsa liphoso tsa batho le ho eketsa bokhoni.
6. Ho lumellana le mefuta e sa tšoaneng ea mehlala: Tekanyetso ea kinetic chromogenic e lumellana le mefuta e mengata ea mehlala, ho akarelletsa le meriana, lisebelisoa tsa bongaka, biologics, le mehlala ea metsi.Ke mokhoa o feto-fetohang o ka sebelisoang liindastering le lits'ebetsong tse fapaneng moo tlhahlobo ea endotoxin e hlokahalang.
Ka kakaretso, tlhahlobo ea kinetic chromogenic endotoxin e fana ka mokhoa o hlokolosi, o potlakileng le o tšepahalang oa ho lemoha le ho lekanya palo.
endotoxins ka lisampole.E sebelisoa haholo indastering ea meriana, biotechnology, le tlhokomelo ea bophelo bakeng sa taolo ea boleng le polokeho.
maikemisetso a kelo.
Nako ea poso: Jul-29-2019
