Teko ea baktheria endotoxin (BET) e etsoa lilaboratoring tse ngata tsa sejoale-joale tlas'a maemo a laoloang e le ntlha ea bohlokoa ho qoba tšitiso.
E loketsengmokhoa oa aseptice bohlokoa ha ho lokisoa le ho hlapolla litekanyetso le ho tšoara lisampole.Ho roalaho sebetsa kantle ho lisebelisoa tse tloaelehileng tsa ts'ireletso ea laboratori (PPE) litlhoko ha se taba ntle le haeba sehlahisoa se tlas'a teko se batla lintlha tse khethehileng tsa polokeho ea mohlahlobi ka lebaka la chefo kapa tšoaetso.Liatlanae lokela ho se be le TALC, kaha TALC e kanna ea ba le maemo a bohlokoa a endotoxins.Li-plate readers, libate tsa metsi, le li-block tsa mocheso o omilenge sebelisoang bakeng sa ho kenya sampole e lokela ho ba bencheng ea laboratori hole le likotopo tsa ho futhumatsa, moea le moea o futhumatsang moea (HVAC), thothomelo e kholo, le sephethephethe sa laboratori se ka amang sephetho sa liteko.Mehlala ea ho ts'oara nako le maemoe lokela ho etsoa qeto le ho ngoloa ka mor'a moo, haeba ho hlokahala, ho netefatsa hore liphetho tse nepahetseng tsa liteko li ka hlahisoa ka nako e loketseng.
Mohlala, haeba laboratori e amohela Metsi a Enteng (WFI) kapa sampole e ntseng e sebelisoa, na e tlameha ho kenngoa ka sehatsetsing kapa na e ka lula mochesong oa kamore, le nako e kae?Pele ho tekolo, ho kgothaletswa hore setshelo(di) sa mantlha sa disampole se kopanngwe ka ho lekaneng pele o tlosa diteko tsa tekolo bakeng sa diteko tse tobileng kapa dilution e latelang.
Teko ea Bioendo Bacterial Endotoxin, liteko li kenyelletsamokhoa oa ho koala geltlhahlobo ea tlhahlobo ea endotoxin letlhahlobo ea tlhahlobo ea endotoxin, mokhoa oa gel clot endotoxin test assay ke tlhahlobo ea boleng ba endotoxin, liteko tsena li hloka hore lisebelisoa ke ts'ebetso ea depyrogenation, joalo ka li-endotoxin free reaction tubes, dilution tubes le malebela a mahala a pyrogen;tlhahlobo ea endotoxin ea bongata e na le tlhahlobo ea kinetic chromogenic endotoxin, tlhahlobo ea kinetic turbidimetric endotoxin, liteko tsena li hloka hore lisebelisoa li kopane le boemo bo holimo ba li-endotoxin tse ka tlase ho.0.005EU/ml( 0.001EU / ml), joalo ka li-tubes tse sa lefelloeng tsa endotoxin, malebela a mahala a pyrogen, le li-microplates tsa mahala tsa pyrogen, esita le sebaka sa mahala sa pyrogen.Ha e le hantle, haeba lisampole li phekoloa, setshelo e lokela ho ba botlolo ea sampole e sa lefelloeng ea endotoxin.
Tekong ea endotoxin, tšitiso e ka hlaha ho tsoa mehloling e fapaneng, joalo ka likarolo tsa mohlala oa matrix, li-reagents tsa liteko kapa lisebelisoa.
Ho qoba tšitiso ea liteko, mehato e latelang e ka nkuoa:
1. Tokiso ea Mohlala: Tokiso e nepahetseng ea sampole e bohlokoa bakeng sa tlhahlobo e nepahetseng ea endotoxin.
Mohlala oa matrix o lokela ho lekoa ka botlalo le ho ntlafatsoa ho netefatsa hore o lumellana le tlhahlobo ea endotoxin.
Haholo-holo, lintho tse sitisang tse kang lipids le liprotheine li lokela ho tlosoa kapa ho fokotsoe ho sebelisoa mekhoa e nepahetseng e kang ho filtration kapa centrifugation.
2. Litaolo tse ntle le tse mpe: Ho bohlokoa ho kenyelletsa litsamaiso tse ntle le tse mpe tekong ea ho beha leihlo bakeng sa tšitiso.
Litaolo tse ntle li tiisa tšebetso ea tlhahlobo, ha li-taolo tse mpe li lemoha tšilafalo leha e le efe kapa tšitiso ho tsoa likarolong tsa tlhahlobo.
3. Taolo ea Boleng: Taolo ea boleng e lokela ho etsoa ho li-reagents tsohle, lisebelisoa, le metsi a sebelisoang tlhahlobong.
Sena se tiisa hore li-reagents ha li na tšoaetso ea endotoxin 'me li sebetsa ka nepo.
4. Boemo: Teko e lokela ho ba ea maemo ho etsa bonnete ba hore liphetho tsohle lia bapisa le hore li ka hlahisoa hape.
Sena se kenyelletsa ts'ebeliso ea curve e tloaelehileng ho lekanya tlhahlobo le ts'ebeliso ea mekhoa e emeng bakeng sa ho lokisa sampole, ho kenya le ho lemoha.
5. Ho netefatsa: Teko e lokela ho netefatsoa ho netefatsa hore e totobetse, e hlokolosi, ebile e ka tšeptjoa.
Sena se kenyelletsa ho lekola lisampole tse ngata, ho kenyeletsoa le tse tsejoang li na le endotoxin, ho fumana bonnete le bonnete ba tlhahlobo.
Ka ho latela mehato ena, tšitiso e ka fokotsoa, 'me ho ka fihleloa tlhahlobo e nepahetseng ea endotoxin.
Nako ea poso: Dec-01-2022
