Ts'ebetsong eatlhahlobo ea baktheria endotoxin, tšebeliso ea metsi a se nang endotoxin ke ea bohlokoa ho qoba tšoaetso.Ho ba teng ha li-endotoxin ka metsing ho ka lebisa ho sephetho se sa nepahalang le ho sekisetsa sephetho sa tlhahlobo.Mona ke moo metsi a reagent ea Lyophilized Amebocyte Lysate (LAL) le baktheria endotoxin test (BET) metsi a kenang teng.Metsi ana a entsoeng ka mokhoa o khethehileng a bohlokoa ho netefatsa ho ts'epahala le ho nepahala ha tlhahlobo ea endotoxin liindastering tse fapaneng, ho kenyeletsoa meriana, lisebelisoa tsa bongaka, lilaboratori tsa lipatlisiso joalo-joalo.
TheMetsi a ts'ebetso ea LALke metsi a hloekisitsoeng haholo a etselitsoeng ka ho khetheha ho sebelisoa tekong ea LAL bakeng sa li-endotoxins.Metsi ana a kena tšebetsong e thata ea tlhahiso ho netefatsa hore ha a na li-endotoxins, tse ka sitisang liphetho tsa liteko.Ho ba sieo ha li-endotoxin ka metsing a reagent ea LAL ho bohlokoa ho netefatsa kutlo le ho nepahala ha tlhahlobo ea LAL, ho e etsa khetho e nepahetseng bakeng sa ho sibolloa ha endotoxin.
Ka mokhoa o ts'oanang, metsi a BET le ona ke karolo ea bohlokoa tekong ea tlhahlobo ea endotoxin ea baktheria.Metsi ana a lokisitsoe le ho lekoa ka ho khetheha ho netefatsa hore ha a na li-endotoxins le litšila tse ling tse ka amang ho nepahala ha tlhahlobo.Ho sebelisa metsi a BET tekong ea tlhahlobo ea endotoxin ke habohlokoa bakeng sa ho fumana liphello tse ka tšeptjoang le tse ka hlahisoang hape, kaha ho felisa kotsi ea ho ba le maikutlo a fosahetseng kapa a fosahetseng a ka hlahang ka lebaka la boteng ba endotoxin metsing a tloaelehileng.
Bohlokoa ba ho sebelisa metsi a se nang endotoxin tekong ea teko ea endotoxin bo ke ke ba feteletsoa.Ho nepahala le ho tšepahala ha liphetho tsa liteko ho itšetlehile ka boleng ba metsi a sebelisoang.Ho ba teng ha li-endotoxin ka metsing ho ka lebisa ho lipalo tse fosahetseng, tse ka bang le litlamorao tse tebileng indastering moo tlhahlobo ea endotoxin e leng bohlokoa bakeng sa ho netefatsa polokeho le katleho ea lihlahisoa.Ka hona, ho tsetela metsing a reagent a LAL kapa metsi a BET ho bohlokoa bakeng sa ho boloka botšepehi ba ts'ebetso ea tlhahlobo ea endotoxin le ho netefatsa boleng le polokeho ea lihlahisoa.
Qetellong, tšebeliso ea metsi a se nang endotoxin, joalo ka metsi a LAL reagent le metsi a BET, a bohlokoa ts'ebetsong ea tlhahlobo ea tlhahlobo ea baktheria endotoxin.Metsi ana a entsoeng ka mokhoa o khethehileng a etselitsoe ho felisa kotsi ea tšoaetso le ho netefatsa ho nepahala le ho tšepahala ha tlhahlobo ea endotoxin.Ka ho sebelisa metsi ana, liindasteri li ka etsa tlhahlobo ea endotoxin ka kholiseho ntle le tšabo ea liphetho tse sa nepahalang ka lebaka la boteng ba li-endotoxin ka metsing.Qetellong, ts'ebeliso ea metsi a reagent ea LAL le metsi a BET a bohlokoa bakeng sa ho ts'ehetsa maemo a holimo ka ho fetisisa a boleng le polokeho liindastering moo tlhahlobo ea endotoxin e leng ea bohlokoa haholo.
Ha u etsa tlhahlobo ea baktheria ea endotoxin, ho bohlokoa ho sebelisa metsi a se nang endotoxin ho netefatsa liphetho tse nepahetseng le tse tšepahalang.
Li-endotoxin ke likarolo tse tsitsitseng tsa mocheso leboteng la lisele tsa baktheria ea gram-negative, 'me li ka baka feberu, ho tšoha, esita le lefu ho batho le liphoofolo.
Ka hona, ho bohlokoa ho sebelisa metsi a se nang li-endotoxins ha u etsa tlhahlobo.
Ho na le mefuta e 'maloa ea metsi e ka sebelisoang tekong ea baktheria endotoxin, ho kenyeletsoa metsi a reagent ea LAL, metsi a reagent a TAL, le metsi a nang le kalafo ea depyrogenation.E 'ngoe le e' ngoe ea mefuta ena ea metsi e etselitsoe ho etsa bonnete ba hore li-endotoxins ha li teng, kahoo ho netefatsa ho nepahala ha liphetho tsa tlhahlobo.
Metsi a reagent a LAL ke metsi a kileng a lekoa ka ho khetheha 'me a netefatsoa hore ha a na li-endotoxins.Metsi ana a atisa ho sebelisoa tekong ea Lyophilized Amebocyte Lysate (LAL), e leng mokhoa o tloaelehileng haholo oa ho lemoha li-endotoxins.Ka ho sebelisa metsi a reagent ea LAL tekong, bafuputsi ba ka kholiseha hore metsi ka boeona ha a fane ka sephetho leha e le sefe se fosahetseng kapa se fosahetseng.
Ka mokhoa o ts'oanang, metsi a ts'ebetso ea TAL ke metsi a kileng a lekoa ka ho khetheha 'me a netefatsoa hore ha a na li-endotoxins.Metsi ana a atisa ho sebelisoa tekong ea Tachypleus Amebocyte Lysate (TAL), mokhoa o mong o tloaelehileng oa ho lemoha li-endotoxins.Ka ho sebelisa metsi a ts'ebetso ea TAL tekong, bafuputsi ba ka kholiseha hore metsi ka boeona ha a fane ka sephetho leha e le sefe se fosahetseng kapa se fosahetseng.
Metsi a nang le kalafo ea depyrogenation ke khetho e 'ngoe ea ho netefatsa hore metsi a sebelisitsoeng tekong ea tlhahlobo ea baktheria endotoxin ha a na li-endotoxins.Kalafo ea depyrogenation e kenyelletsa ho tlosoa kapa ho se sebetse ha li-pyrogens, ho akarelletsa le endotoxins, metsing.Sena se ka finyelloa ka mekhoa e kang filtration, distillation, kapa phekolo ea lik'hemik'hale.Ka ho sebelisa metsi a nang le phekolo ea depyrogenation tekong, bafuputsi ba ka kholiseha hore metsi ka boeona ha a kenye letsoho liphellong leha e le life tse fosahetseng kapa tse fosahetseng.
Joale, ke hobane'ng ha ho le bohlokoa hakaale ho sebelisa metsi a se nang endotoxin tekong ea tlhahlobo ea baktheria endotoxin?Ho ba teng ha li-endotoxin ka metsing a sebelisoang tlhahlobong ho ka lebisa liphellong tse sa nepahalang, tse ka bang le liphello tse tebileng bakeng sa lipatlisiso le likopo tsa bongaka.Ka mohlala, haeba li-endotoxins li le teng metsing, li ka lebisa liphellong tse fosahetseng tsa bohata, tse bontšang boteng ba li-endotoxins ha li hlile li le sieo.Sena se ka lebisa matšoenyehong a sa hlokahaleng le tšebeliso e mpe ea lisebelisoa ho rarolla bothata bo seng teng.
Ka lehlakoreng le leng, haeba li-endotoxins li le teng ka metsing 'me li sa bonoe, li ka lebisa liphellong tse fosahetseng tsa bohata, tse bontšang hore li-endotoxin ha li teng ha li hlile li le teng.Sena se ka lebisa ho lokolloeng ha lihlahisoa tse silafetseng, ho beha bophelo ba batho le liphoofolo kotsing.
Ntle le tšusumetso e ka bang teng mabapi le ho nepahala ha liphetho tsa liteko, ho sebelisa metsi a se nang endotoxin le hona ho ka ama ts'ebetso ea tlhahlobo ka boeona.Li-endotoxins li ka kena-kenana le li-reagents le lisebelisoa tse sebelisoang tekong, e leng se lebisang ho liphello tse sa tšepahaleng kapa tse sa lumellaneng.Ka ho sebelisa metsi a se nang endotoxin, bafuputsi ba ka fokotsa likotsi tsena le ho netefatsa hore tlhahlobo e etsoa tlas'a maemo a tšepahalang ka ho fetisisa.
Qetellong, ho netefatsa hore metsi a sebelisitsoeng tekong ea tlhahlobo ea baktheria endotoxin ha a na li-endotoxins ho bohlokoa bakeng sa ho boloka botšepehi ba liphetho tsa liteko.Ho sa tsotellehe hore na ba sebelisa metsi a LAL reagent, TAL reagent water, kapa metsi a nang le phekolo ea depyrogenation, bafuputsi ba ka nka mehato ea ho etsa bonnete ba hore metsi ha a kenye letsoho ho se nepahaleng kapa ho se lumellane ha liphello tsa tlhahlobo.Ka ho etsa joalo, ba ka ba le kholiseho mabapi le bonnete ba liphuputso tsa bona mme ba ka etsa liqeto tse nang le tsebo ho latela liphetho tsa tlhahlobo.
Nako ea poso: Jan-26-2024
