Malebela a pipette a se nang pyrogen - Endotoxin mahala test tubes / tips / microplates

Lisebelisoa tse se nang pyrogen ke lintho tse ka jeoang ntle le endotoxin ea kantle, ho kenyeletsoa malebela a pipette a se nang pyrogen (lebokose la ntlha), li-tubes tsa tlhahlobo tse se nang pyrogen kapa tse bitsoang li-endotoxin mahala tsa khalase, li-ampoules tsa khalase tse se nang pyrogen, li-microplates tse se nang endotoxin tse 96, le endotoxin- metsi a sa lefelloeng (tšebeliso ea metsi a depyrogenated tekong ea baktheria endotoxin), Endotoxin mahala buffer le joalo-joalo Har'a tsona, metsi bakeng sa baktheria endotoxintest ka mokhoa oa ho koala gel le tlhahlobo ea tlhahlobo ea tlhahlobo ea endotoxin likhatisong tsohle tse amanang le Pharmacopoeia (USP, EP, BP, JP le China Pharmaceutica). E bua ka metsi a hloekisitsoeng bakeng sa ente e nang le endotoxin e ka tlase ho 0.015EU/ml. Hona joale mofuta oa morao-rao oa Pharmacopoeia, metsi a BET a ka tlase ho 0.005EU/ml. Esita le boemo bo phahameng ka ho fetisisa bo ka tlaase ho 0.001EU/ml bo khona ho hlahisoa le ho fanoa ke Bioendo.

Tlhaloso e fana ka tlhaloso e qaqileng ea lisebelisoa tse se nang pyrogen le lisebelisoa tsa tsona, haholo-holo ho hatisa karolo ea bona ea bohlokoa tekong ea endotoxin ho pholletsa le litekanyetso tse fapaneng tsa pharmacopoeia. Kakaretso ea lintlha tsa bohlokoa ke tsena:

1. Tlhaloso:
   • Lisebelisoa tse se nang pyrogen ke lintho tse se nang li-endotoxin tse sa tloaelehang, ho kenyelletsa:
     • Malebela a pipette a se nang pyrogen(lebokose la ntlha).
     • Li-tubes tsa tlhahlobo tse se nang endotoxin kapa li-tubes tsa khalase.
     • Li-ampoules tsa khalase tse se nang endotoxin.
     • Li-microplate tse se nang endotoxin tse se nang 96-liliba.
     • Metsi a se nang endotoxin (metsi a depyrogenated).
     • Li-buffer tse se nang endotoxin.
2. Metsi bakeng sa Teko ea Baktheria Endotoxin (BET):
   • E sebelisoa mekhoeng ea ho koala li-gel le liteko tsa palo ea endotoxin ho latela litekanyetso tsa USP, EP, BP, JP, le China Pharmacopeia.
   • Tekanyetso ea pejana: ≤0.015 EU/ml bakeng sa metsi a nyopa bakeng sa ente.
   • Tlhokahalo ea hajoale (pharmacopoeia ea morao-rao): ≤0.005 EU / ml bakeng sa metsi a BET.
   • Tekanyetso e tsoetseng pele: Bioendo e ka hlahisa metsi a nang le ≤0.001 EU/ml endotoxin content, e finyellang litlhoko tsa boleng bo phahameng ka ho fetisisa.

Litlhahiso tsa ho ntlafatsa:

• Hatisa boholo ba ts'ebeliso, mohlala, lilaboratori, meriana, kapa biotech.
• Fana ka lintlha tse ling mabapi le boitseanape ba Bioendo ho etsa tlhahiso ho matlafatsa tlholisano ea eona.
• Totobatsa melemo e khethehileng ea lihlahisoa tsa maemo a holimo tsa Bioendo, joalo ka ho nepahala ha tlhahlobo e ntlafalitsoeng kapa ho latela melao.

Na o ka rata hore ke u thuse ho etsa sehlahisoa se hlophisitsoeng haholoanyane kapa tlhaloso ea tekheniki?

Mantsoe a bohlokoa a amanang le li-endotoxins, le lisebelisoa tsa mahala tsa endotoxin, mehloli ea pyrogen le mocheso ke likhopolo tse peli tse fapaneng ka ho feletseng:Pyrogen: E boetse e bitsoa pyrogen kapa exothermic factor. dintho tse ka bakang keketseho ya mocheso wa mmele.Mohloli oa Mocheso: Ntho e ntshang mocheso. Tse kang ho chesa lipapali, mashala, joalo-joalo "Lisebelisoa tse seng tsa pyrogenic" le "karabelo ea pyrogenic" ea bahlahisi ba bang le bahoebi ha e le hantle ke mabitso a sa rutehang haholo le a khelosang. Tse nepahetseng li lokela ho ba "Pyrogen Free" le "Pyrogen Response".

Hobaneng ha lisebelisoa tsa mahala tsa Pyrogen li hlokahala tekong ea tlhahlobo ea endotoxin, tlhahlobo ea tlhahlobo ea gel clot endotoxin le tlhahlobo ea tlhahlobo ea endotoxin?

E, lisebelisoa tse se nang pyrogen ke tsa bohlokoa bakeng sa ho etsa tlhahlobo ea tlhahlobo ea endotoxin ka nepo le ka botšepehi.Ho ba teng ha li-pyrogens, e leng lintho tse bakang feberu hangata tse tsoang ho li-endotoxin tsa baktheria, li ka kena-kenana le liphello tsa tlhahlobo le ho lebisa ho baleng ba bohata. tlhahlobo ea endotoxin, eo hangata e tsejoang e le tlhahlobo ea Limulus amebocyte lysate (LAL) kapa e bitsoang Lyophilized tlhahlobo ea amebocyte lysate (LAL), e sebelisetsoa ho bona le ho lekanya boteng ba li-endotoxin tsa baktheria litlhare, lisebelisoa tsa bongaka le lihlahisoa tse ling. Tlhahlobo ea LAL e itšetlehile ka karabelo pakeng tsa LAL reagent le endotoxins ho hlahisa karabo ea ho koala kapa ea chromogenic. Ho netefatsa liphello tse nepahetseng, ho bohlokoa ho sebelisa lisebelisoa tse se nang li-pyrogens. Li-pyrogens li ka silafatsa lisebelisoa tse fapaneng tsa laboratori, ho kenyeletsoaglassware, malebela a pipette, li-tubes, le lijana tsa mohlala. Haeba lisebelisoa tse silafalitsoeng ke pyrogen li kopana le reagent ea LAL kapa disampole tsa liteko, li ka tsosa maikutlo a fosahetseng, e leng se lebisang ho liqeto tse fosahetseng mabapi le boteng kapa bongata ba li-endotoxins. boteng ba li-pyrogens. Ba kena lits'ebetsong tse matla tsa taolo ea boleng ho netefatsa hore ba fihlela litekanyetso tse hlokahalang bakeng sa tlhahlobo ea endotoxin. Ho sebelisa lisebelisoa tsena tse khethehileng ho thusa ho boloka botšepehi le ho nepahala ha tlhahlobo ea tlhahlobo ea endotoxin, ho netefatsa liphetho tse tšepahalang le ho khothaletsa polokeho ea mokuli lits'ebetsong tsa meriana le tsa biomedical.