Li-tubes tsa khalase tse nang le ts'ebetso ea depyrogenation lia hlokahala tekong ea tlhahlobo ea endotoxin ho netefatsa bonnete le ts'epahalo ea liphetho tsa liteko.Li-endotoxins ke likarolo tsa limolek'hule tse tsitsitseng mocheso tsa lerako la lisele tse ka ntle tsa libaktheria tse ling tsa gram-negative, 'me li ka baka boloetse bo tebileng esita le lefu ho batho haeba li le teng lihlahisoa kapa lisebelisoa tsa bongaka.
Ho lemoha li-endotoxins, tlhahlobo e sebelisa li-reagents tse nang le Limulus Amebocyte Lysate (LAL) kapa tse bitsoang Lyophilized amebocyte lysate, e leng karolo ea lisele tsa mali tsa horseshoe tse nang le mochine oa ho koala o sebelisoang ke li-endotoxins.Leha ho le joalo, li-tubes tsa khalase tse sa kang tsa senyeha li ka kena-kenana le tlhahlobo ea LAL ka ho kenya mochine oa eona oa ho koala le ho hlahisa liphello tse fosahetseng.Ka hona, li-tubes tsa khalase tse sebelisitsoeng tekong ea tlhahlobo ea endotoxin li tlameha ho tlosoa ho tlosa li-endotoxin tse ka bang teng le ho thibela ts'ebetso ea LAL reagent.Sena se tiisa hore liphetho tsa tlhahlobo ea endotoxin li nepahetse ebile lia tšepahala le hore bakuli ha ba pepesehetse maemo a kotsi a endotoxin.le ho netefatsa polokeho ea lithethefatsi tsa botsoali litlhare, liprotheine, setso sa lisele, DNA joalo-joalo.
Bohlokoa ba ho sebelisa li-tubes tsa khalase tse se nang endotoxin ts'ebetsong ea tlhahlobo ea tlhahlobo ea endotoxin:
Li-tubes tsa khalase tse se nang endotoxinke karolo ea bohlokoa ea tlhahlobo efe kapa efe ea tlhahlobo ea endotoxin.Li-tubes tsena tsa khalase li etselitsoe ho fokotsa kotsi ea tšilafalo ea endotoxin nakong ea tlhahlobo, ho netefatsa liphetho tse nepahetseng le tse tšepahalang.
E 'ngoe ea likarolo tsa bohlokoa tsa li-tubes tsa khalase tse se nang endotoxin ke sebopeho sa tsona sa lik'hemik'hale.Li-tubes tsena li entsoe ka khalase ea boleng bo holimo ea borosilicate, e tsejoang ka ho hanyetsa ha eona ka tsela e ikhethang khahlanong le ho bola ha lik'hemik'hale.Sena se etsa hore e be tse loketseng bakeng sa ho sebelisoa tlhahlobong ea endotoxin, kaha li khona ho mamella ho pepesetsoa metsoako e mengata ea liteko ntle le ho nyenyefatsa kapa ho silafatsa sampole.
Ntho e 'ngoe ea bohlokoa ea li-tubes tsa khalase tse se nang endotoxin ke bohloeki ba tsona.Li-tubes tsena li hloekisoa ka hloko pele li sebelisoa ho tlosa lisosa tse ka bang teng tsa tšoaetso.Li boetse li lekoa ka matla bakeng sa tšilafalo ea endotoxin, ho etsa bonnete ba hore ha li na mesaletsa ea ntho ena e kotsi.
Ho feta moo, li-tubes tsa khalase tse se nang endotoxin li etselitsoe ho ba bonolo ho li sebelisa.Ka tloaelo li fumaneha ka mefuta e fapaneng ea boholo le litlhophiso ho amohela mefuta e fapaneng ea sampole le mekhoa ea tlhahlobo ea tlhahlobo ea boleng ba endotoxin le tlhahlobo ea tlhahlobo ea endotoxin.Li boetse li tsamaisana le mefuta e fapaneng ea lisebelisoa tsa ho lokisa le ho etsa liteko, e leng se etsang hore e be khetho e feto-fetohang le e bonolo bakeng sa lilaboratori tsa tlhahlobo ea endotoxin.
Ka kakaretso, li-tubes tsa khalase tse se nang endotoxin li bapala karolo ea bohlokoa ho nepahala le ho ts'epahala ha tlhahlobo ea endotoxin.Kaho ea tsona ea boleng bo holimo, bohloeki, le boiketlo ba ts'ebeliso li li etsa karolo ea bohlokoa ea tlhahlobo efe kapa efe e atlehileng ea tlhahlobo ea endotoxin.
Li-tubes tsa khalase tse se nang Bioendo Endotoxin tse nang le boholo10*75mm, 12*75mm, 13*100mm le 16*100mmbakeng sa mekhoa ea dilutions le mekhoa ea karabelo.
Li-tubes tsa khalase tse se nang endotoxin li fihlella maemo a holimo a li-endotoxin tse ka tlase ho 0.005EU/ml.
Li-tubes tsa khalase tse se nang endotoxin li lokela ho sebelisoa tekong ea tlhahlobo ea gel clot endotoxin ho thibela liphetho tse fosahetseng.
Li-endotoxins ke likarolo tsa lebota la baktheria tse ka silafatsang lisebelisoa tsa laboratori, ho kenyeletsoa le li-tubes tsa khalase.
Teko ea tlhahlobo ea gel clot endotoxin e sebelisoa ho bona boteng ba li-endotoxin sampuling.Tekong ena, lesela le thehoa boteng ba li-endotoxins.Sebopeho sena sa lesela se bapisoa le taolo ea ho tseba hore na endotoxin e hokae.
Ho sebelisa li-tubes tsa khalase tse se nang endotoxin ho thusa ho netefatsa hore tlhahlobo ea endotoxin e nepahetse.Lebaka ke hobane li-endotoxins li ka khomarela bokaholimo ba li-tubes tsa khalase mme tsa kena-kenana le liphetho tsa tlhahlobo.
Ho etsa bonnete ba hore li-tubes tsa khalase tse sebelisoang tekong ea tlhahlobo ea gel clot endotoxin ha li na endotoxin, li lokela ho hlatsuoa ka sesepa ebe li hlatsuoa hantle ka metsi a se nang endotoxin.Ho feta moo, li lokela ho hloekisoa ka ho sebelisa autoclaving kapa sterilization ea mocheso o omileng.
Qetellong, ho sebelisa li-tubes tsa khalase tse se nang endotoxin tekong ea tlhahlobo ea gel clot endotoxin ho bohlokoa ho netefatsa ho fumanoa ka nepo ha li-endotoxin.Li-tubes tsena li lokela ho hloekisoa hantle le ho hloekisoa ho felisa tšilafalo leha e le efe e ka bang teng.
Nako ea poso: Jun-02-2023
