Mokhoa oa Chromogenic ke o mong oa mekhoa e meraro e nang le mokhoa oa gel-clot le mokhoa oa turbidimetric oa ho bona kapa ho lekanya li-endotoxin ho tsoa ho baktheria ea Gram-negative ka ho sebelisa amoebocyte lysate e ntšitsoeng maling a maputsoa a makhala a pere (Limulus polyphemus kapa Tachypleus tridentatus).E ka hlalosoa e le tlhahlobo ea "endpoint-chromogenic" kapa "kinetic-chromogenic assay" ho latela molao-motheo oa tlhahlobo o sebelisitsoeng.
Molao-motheo oa karabelo ke hore: amebocyte lysate e na le cascade ea li-enzyme tsa serine protease (proenzymes) tse ka sebelisoang ke li-endotoxin tsa baktheria.Li-endotoxins li kenya tšebetsong li-proenzyme ho hlahisa li-enzyme (tse bitsoang coagulase), ea morao-rao e baka karohano ea karoloana e se nang 'mala, e lokolla sehlahisoa sa pNA se 'mala o mosehla.PNA e lokollotsoeng e ka lekanyetsoa ka mokhoa oa photometric ho 405nm.'Me monyetla o amana hantle le khatello ea endotoxin, joale mahloriso a endotoxin a ka baloa ka nepo.
Nako ea poso: Sep-29-2019
