Gel Clot Lyophilized Amebocyte Lysate Multi-test Vial G17
Gel Clot Lyophilized Amebocyte Lysate (LAL) Multi-test Vial, G17 series
1. Tlhahisoleseding ea Sehlahisoa
Gel Clot Lyophilized Amebocyte Lysate Multi-test Vial ke Lyophilized Amebocyte Lysate reagent e khethang mokhoa oa ho koala gel ho lemoha endotoxin kapa pyrogen.Joalo ka mokhoa o atileng, tlhahlobo ea gel-clot bakeng sa endotoxin e bonolo ebile ha e hloke lisebelisoa tse khethehileng le tse turang.Bioendo e fana ka Gel Clottlhahlobo ea endotoxinkit ka 1.7ml ka vial.
2. Parameter ea sehlahisoa
Sensitivity range: 0.03EU/ml, 0.06EU/ml, 0.125EU/ml, 0.25EU/ml, 0.5 EU/ml
3. Kopo ea Sehlahisoa
Sehlahisoa sa ho qetela sa endotoxin (pyrogen), metsi bakeng sa entetlhahlobo ea endotoxin, lisebelisoa tse so ntlafatsoetlhahlobo ea endotoxinkapa tlhahlobo ea boemo ba endotoxin nakong ea ts'ebetso ea tlhahiso bakeng sa lik'hamphani tsa meriana kapa baetsi ba lisebelisoa tsa bongaka.
Tlhokomeliso: Lyophilized Amebocyte Lysate (LAL reagent) e entsoeng ke Bioendo e entsoe ka lysate ea amebocyte (lisele tse tšoeu tsa mali) tse tsoang ho khala ea pere.
Mokhoa oa ho koala gelSetšoantšo sa LAL, reconstituted lysate reagent fumana bonyane liteko tse 16 ka vial:
Nomoro ea lethathamo | Sensitivity (EU/ml kapa IU/ml) | ml/botlolo | Liteko/Vial | Linkho/Pakete |
G170030 | 0.03 | 1.7 | 16 | 10 |
G170060 | 0.06 | 1.7 | 16 | 10 |
G170125 | 0.125 | 1.7 | 16 | 10 |
G170250 | 0.25 | 1.7 | 16 | 10 |
G170500 | 0.5 | 1.7 | 16 | 10 |
Boemo ba sehlahisoa:
The Lyophilized Amebocyte Lysate reagent sensitivity le Control Standard Endotoxin potency li lekoa khahlanong le USP Reference Standard Endotoxin.Lisebelisoa tsa reagent tsa Lyophilized Amebocyte li tla le litaelo tsa sehlahisoa, Setifikeiti sa Analysis.
E 'ngoe ea likarolo tsa bohlokoa tsa letoto la Bioendo Lysate reagent G17 ke khanyetso ea eona e matla ea tšitiso.Sena se bohlokoa haholo ha o sebetsa ka boholo ba lisampole tse kholo, kaha ho na le monyetla o moholo oa tšitiso e tsoang ho lintho tse ling tse teng sampoleng.Theho fumanoa ha endotoxints'ebetso e hloka tekanyo e phahameng ea kutlo le ho khetheha, 'me reagent ea Bioendo Lysate e entsoe ka ho khetheha ho fokotsa tšusumetso ea lintho tse sitisang.Sena se tiisa hore ts'ebetso ea ho lemoha endotoxin e nepahetse ebile e ka tšeptjoa, leha e sebetsa ka boholo ba sampole.
Ntle le ho hanyetsa ha eona ho kena-kenana, Gel Clot Method Lyophilized Amebocyte Lysate Multi-test Vial G17 e boetse e etselitsoe hore e sebelisoe habonolo.Sebopeho sa lyophilized sa reagent se lumella ho tsitsa ha nako e telele le ho boloka habonolo, ha mokhoa oa li-vial oa mefuta e mengata o etsa hore ho be bonolo ho etsa liteko tsa mehlala e mengata.Sena se etsa hore reagent ea Bioendo Lysate e be khetho e sebetsang le e theko e tlaase bakeng sa ho lemoha endotoxin lits'ebetsong tse kholo, tse kang tlhahiso ea meriana le tlhahiso ea lisebelisoa tsa bongaka.Ka kakaretso, theTlhahlobo ea Gel Clot LAL, ho sebelisa reagent ea Bioendo Lysate, ke sesebelisoa sa bohlokoa sa ho netefatsa polokeho le boleng ba lihlahisoa ka ho lemoha tšilafalo ea endotoxin ka bongata.
Hobaneng ha li-microbiologists li khetha letoto la tlhahlobo ea gel clot assay G17?
1. LAL reagent G17 letoto ke mokhoa o akaretsang ka ho fetesisa oa ho bonts'a boteng ba li-endotoxins tse ipapisitseng le hore na ho etsoa ha gel ka har'a tube ea sampole ha ho sebetsa.tlhahlobo ea baktheria endotoxin.
2. Tse ngatatlhahlobo ea limulus lysatebakeng sa ho lemoha endotoxin ho lithethefatsi tsa batsoali, ha ho fumanoa lisampole tsa bongata bo itekanetseng.
3. Mokhoa oa tlhahlobo oa LAL oa ho incubation, sesebelisoa se loketseng ke ho hlapa ka metsi kapa mochine o omileng oa mocheso.
4. Boleng bo phahameng ba ho qetela ba endotoxin mahala tube (<0.005EU/ml) le Boleng bo phahameng ba pyrogen free tip (<0.005EU/ml) e le lisebelisoa tse tiisitsoeng ho netefatsa sephetho se nepahetseng.
Lihlahisoa tse amanang tekong ea tlhahlobo ea endotoxin:
- Metsi bakeng sa Teko ea Baktheria Endotoxins (BET), Khothalletsa TRW50 kapa TRW100
- Endotoxin mahala khalase tube ( dilution tube ), Khothalletsa T1310018 le T107540
- Malebela a mahala a pyrogen, Recommend PT25096 or PT100096
- Pipettor, Khothalletsa PSB0220
- Test Tube Rack
- Incubation Instrument (Bath ea Metsi kapa Dry Heat Incubator ), Recomet Dry Heat Incubator TAL-M2
- Motsoako oa Vortex, Khothalletsa VXH.
- Laola Endotoxin e Tloaelehileng, CSE10V.
BioendoTlhahlobo ea LAL bakeng sa li-pyrogens or Tlhahlobo ea LAL endotoxin, leTlhahlobo ea TAL, litlhaloso tsohle ke tsa tlhahlobo ea endotoxin.
Ho ipapisitsoe le bonono ba ts'ebetso ea Bioendo, lisebelisoa ke lyophilized amebocyte lysate (LAL).
Limulus amebocyte lysate (LAL) ke karolo ea aqueous ea lisele tsa mali (li-amoebocyte) ho tloha hoCrab ea Atlantic horseshoe Limulus polyphemus.LAL e sebetsana le baktheriaEndotoxin lipopolysaccharide(LPS), e leng alerakarolo ealibaktheria tsa gram-negative.Karabelo ena ke motheo oaTlhahlobo ea LAL, e sebelisoang haholo bakeng sa ho lemoha le ho lekanya palo ea baktheriali-endotoxins.
Naheng ea Asia, ho joaloTachypleus amebocyte lysate (TALA) teko e thehiloeng holim'a makhala a sebaka sa pereshoeTachypleus gigas or Tachypleus tridentatuse sebelisoa ka linako tse ling.Therecombinant factor C(rFC) tekolo ke aphetohoea LAL/TAL e ipapisitse le maikutlo a tšoanang.